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Description

The neutrophilic inflammatory phenotype is associated with systemic inflammation in asthma

Murine NIH 3T3 fibroblasts were originally obtained from ATCC and were cultured in Dulbecco's modified Eagle medium (DMEM) (Invitrogen) supplemented with 10% (vol/vol) FCS, 100 U/mL penicillin, and 100 g/mL streptomycin