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Clinical Anatomy, 28(4):527-37 Cesmebasi A, Muhleman MA, Hulsberg P, Gielecki J, Matusz P, Tubbs RS, Loukas M (2015)

In short, mainly based on both the homology with yeast HDACs and their function, human HDACs are divided in four classes

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Usually we recommend washing the beads in a nonionic detergent like Tween 20 detergent before doing the experiment