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The latter category revealed strong upregulation of genes associated with ISR, including amino acid synthesis and one-carbon metabolism ( Mthfd2 , Pycr1 , Asns , Psat1 ), tRNA charging, amino acid transporters ( Slc7a1 , Slc1a4 , Slc3a2 ) and various stress-response genes ( Fgf21 , Gdf15 , Ddit3 , Trib3 , Atf5 , Atf6 ) (Fig