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For FA studies, M1-polarized macrophages were seeded (50,000 cells per well) and treated with either FA 24:1 (50 M, complexed with BSA) or a BSA vehicle control for 18 h prior to the assay

Cyanocobalamin is the most widely available synthetic form, is shelf-stable, and is FDA-approved for treating B12 deficiency

Vitamin B12 (Cobalamin) Food sources: Fish, meat, poultry, eggs, milk, and other dairy products

Symptoms of deficiency include fatigue, weakness, neurological problems (such as paraesthesia, balance difficulties, and cognitive impairment), and megaloblastic anaemia

Fatigue One of the initial symptoms of vitamin B12 deficiency is feeling tired

Long-term Safety and Monitoring The break serves as a safety buffer to monitor for any latent side effects