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The reactions were carried out in 384 well PCR plates in a total volume of 4 l containing 10 M of reverse and forward primers, 2 l of SensiFAST Sybr lo ROX RT-PCR mix (Bioline, Boston, MA), and 5 ng of template cDNA (1 l)

TABLE 1 Causes of vitamin B12 deficiency, classified by cause

Some items were reverse coded

Its strong safety profile and effective absorption make it suitable for a wide range of patients, including those with poor dietary intake, malabsorption issues, or conditions affecting intrinsic factor

However, if your body has trouble absorbing nutrients (which is common after certain GI conditions or surgeries), your provider may recommend B12 injections

Therefore, we always recommend professional training before anyone administers your shot, well, or any type of B12 injection