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For initial evaluation of the effects of severe hypoxia on E GSH in the ER, we exposed leaf disks of WT plants expressing Grx1- roGFP2iL-HDEL to an atmosphere with only 0.1% O 2 for 1, 3.5 or 6.5 h and followed the sensor response by monitoring the fluorescence excited at 400 nm and 480 nm, respectively, as well as the resulting 400 nm / 480 nm fluorescence ratio (Supplemental Figure S8)