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Endotoxin levels are determined by measuring the activity of Factor C in the presence of the colorless chromogenic synthetic peptide substrate (Ac-Ile-Glu-Ala-Arg-pNA) that releases p-nitroaniline (pNA) after proteolysis, producing a yellow color that can be measured at an absorbance of 405 nm

For this, we used siRNA technology to deplete RECQ1, MUS81, LIG4 or ELL in U2OS cells transduced with the HRASV12 or cyclin E1 retroviral vectors

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In vivo, LA exists as free and protein-bound (predominant form) and is formed by the substitution of sulfur groups for hydrogen at positions 6 and 8 of octane groups [26]

Wu XW, Muzny DM, Lee CC, Caskey CT