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Microbiota-derived short-chain fatty acids functions in the biology of B lymphocytes: From differentiation to antibody formation

PKC - Protein kinase C

Chemical shifts were referenced to the internal 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) signal for 1H 1D spectra

doi: 10.1093/infdis/jiad337 48 LvYChenDTianXXiaoJXuCDuLet al

The immune system plays a major antitumor role Cancer cells undergoing metabolism reprogramming interact with immune cells to disrupt normal immune function 142

For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C