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The in vitro component demonstrated that both glucagon and Retatrutide directly decreased ANGPTL3/8 secretion from hepatocytes, and this effect was blocked by a glucagon receptor (GCGR) antagonist

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Radiolabeled proline (L-[ 3 H]Pro), serine (L-[ 3 H]Ser), glutamine (L-[ 3 H]Gln), asparagine (L-[ 3 H]Asn), leucine (L-[ 3 H]Leu), and glutamate (L-[ 3 H]Glu) (Perkin Elmer) (3 Ci/mL) were used to assess mitochondrial amino acid uptake by the addition of labeled amino acids to the respective tubes, followed by incubation at 37 C for one h

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