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For quantitation of mitochondrial potential and mass of FoB and MZB, splenocytes were stained with 100 nM MitoTracker Deep Red FM and 10 nM MitoTracker Green FM (Fisher Scientific 15754272 and 15784272) as follows

The specific method depends on the condition being treated and the practitioners approach

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Mol Carcinog 37 : 110119

Again, correct insertion of the KEIO fragments in the marker free double mutants was verified using colony PCR with appropriate primers (Table 3)