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The culture broth was subjected to liquidliquid extraction with EtOAc in a 1:2 ratio at room temperature, with stirring at 100 rpm for 72 h

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Following HMGB1 inhibition or 5-HT7R knockout, the downstream Nrf2/xCT/GPX4 signaling pathways was upregulated, resulting in alleviated depressive-like behaviors (Du et al., 2025)

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