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In another aspect of the invention, one or more NNMT inhibitors (such as a cation of Formula I or IA, or otherwise disclosed herein) is contacted with a cell expressing NNMT followed by contacting said cell expressing NNMT with one or more chemical entities (e.g., nicotinamide riboside, nicotinamide mononucleotide) that modulate intracellular NAD+ levels to provide muscular therapy

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[1] [3] The injections work by bypassing the digestive system entirely, delivering B12 directly into muscle tissue where it can be absorbed into the bloodstream

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Although deletion of ABC transporter and isocitrate lyase hinders the capacity of mutant to persist in calf tissue evaluated at the end point, it does not change their ability to induce immune responses compatible with wild-type strain