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Keep in mind that it can take time, such as several weeks, for a deficient cat to have the right amount of B12 in their body again

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Pfmdr1 Copy Number For all genotyping assays, DNA was extracted from FTA filter paper blots or whole blood (for ex vivo specimens) according to manufacturer instructions (QIAamp DNA Blood Mini Kit, QIAGEN, Inc, Alameda, California, USA)

The column was washed with lysis buffer (5 mM imodazole in lysis buffer) and increasing concentrations of NaCl (0.5 mM and 1 mM) followed by increasing concentrations of imidazole (5 mM and 20 mM, in lysis buffer) to remove contaminating proteins

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knockdown of the NNMT protein in the adipose tissue and liver using antisense oligonucleotides limited body weight gain in mice fed high fat diet, causing substantial fat mass reduction via increased energy expenditure