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Chromatographic separation was then performed at 600nL/min using a 60-min linear gradient that ramped solution B (acetonitrile with 0.1% formic acid) from 3% to 32%

therefore, intake of 5-MTHF cannot lead to high accumulation levels in the blood [23]

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Only the LAL assay detects them

These findings align with the GSEA analysis results mentioned above, where enhanced interferon signaling pathways significantly promoted inflammatory cell infiltration and synovial tissue damage (36, 39, 40)