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Description

J Bacteriol 133:11261129 Fujio T, Hayashi M, Tomiyoshi Y, Ozaki A (1985) Compound from its precursor using the enzymic activity of a bacterium

MurD: to 100 mM Tris buffer pH 7.9, 2.0 mM of one UDP-MurNAc-L-Ala derivative ( 1c 3c , Supplementary Information), 4.0 mM D-Glu, 4.0 mM ATP and 2.0 mM MgCl 2 was added 1.0 g purified MurD enzyme per 100 l reaction sample