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coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)

At higher temperatures there was less disruption in the RLS signal compared to that of TaC, and subsequently less vaporisation during melting

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1 Standardized mean differences of covariates in incident GLP-1RA compared to sulfonylureas, DPP4i, SGLT2i, a control composited of equal proportion of incident sulfonylureas, DPP4i, SGLT2i use and a control of participants who received usual care before and after weighting