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Isolation of human PBMCs Buffy coat samples from six healthy, male volunteers were obtained from the blood donation service at the University Hospital of Leipzig, Germany

doi:10.1074/jbc.272.52.32715

Smears typically show agglutinated cells unless the blood tube was previously warmed

elegantly demonstrated that proliferating cells ferment glucose to lactate when shuttle flux becomes saturated 186

On day 0, blood samples were collected via anesthesia-induced cardiac puncture and stored for plasma glycerol analysis

Epidemiological evidence indicates that low bone density in patients with OA is closely related to abnormal bone remodeling