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The slides were washed in water and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer

Computing was supported via the Advanced Research Computing facility, Oxford, the ARCHER UK National Supercomputing Service and JADE (EP/T022205/1) granted via the High-End Computing Consortium for Biomolecular Simulation, (HECBioSim - supported by EPSRC (EP/R029407/1) to P.C.B

It carries copper into cells where copper-dependent enzymes need it, and the GHK fragment itself acts as a signaling peptide that influences gene expression in fibroblasts and other tissue cells

Association of cerebral spinal fluid copper imbalance in amyotrophic lateral sclerosis

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