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At the conclusion of the study, animals were euthanized with a lethal dose of sodium pentobarbital (150 mg/kg, IP), and death was confirmed by the absence of heartbeat and corneal reflex

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P2X7 receptor-mediated purinergic signaling promotes liver injury in acetaminophen hepatotoxicity in mice

After adapter trimming with Trim Galore and quality assessment with FastQC, reads were mapped to the human genome (hg38) using Bowtie2 with default paired-end settings

For the health of the general population, the subtle changes in insulin, GH and IGF-I signaling associated with gene polymorphisms or epigenetic changes programmed during fetal and early postnatal life and affecting gene expression are important