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Proc Natl Acad Sci U S A 117(35):2142021431

Mixed Agonist/Antagonist and Partial Agonist Opioid Analgesics The concomitant use of opioids with other opioid analgesics, such as butorphanol, nalbuphine, pentazocine, may reduce the analgesic effect of acetaminophen and codeine phosphate tablets and/or precipitate withdrawal symptoms

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Then, percent inhibition for each compound tested is calculated using the equation: [0474] The % inhibition data is plotted as a function of test compound concentration and test compound IC 50 is determined using a linear regression in which x is In(concentration of test compound) and y is In(percent inhibition/(100 - percent inhibition)