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After stimulation, cells were washed once with 1x PBS and then collected in 150 l 2x Laemmli Sample Buffer supplemented with 5% 2-Mercaptoethanol

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Injection Technique General subcutaneous technique, following established clinical best-practice guidance [14] [15]

Crucially, the specific papers tying Dihexa to that axis were retracted in 2025 for falsified/fabricated figures, so the mechanism must be treated as unproven pending independent replication [4]

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Emotional sensitivity Increased emotional responsiveness or mood fluctuations may occur as hippocampal connectivity changes