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10.1016/j.brainresbull.2014.04.008 Brain Res

The absorbance of the supernatant was determined at 400 nm

doi: 10.1038/s41538-023-00197-z 10

Pyridoxal phosphate accepts amino groups from aspartate catalyzed by aspartate aminotransferase, and alanine catalyzed by alanine aminotransferase to form Schiffs base intermediates that are subsequently reduced to form pyridoxamine phosphate

Gel filtration chromatography Recombinant PGRMC1 (a.a.44195) (10 g) wt, Y113F or C129S mutant, treated with 5 mmol l 1 sodium dithionite and/or CO gas or left untreated, was separated on a Superdex 200 column equilibrated in buffer containing 20 mmol l 1 Tris-HCl (pH 7.5) and 100 mmol l 1 NaCl using a SMART system (GE Healthcare)

2.0 system, Blk -CH blocking buffer ( EMD Millipore ) was added and the vacuum immediately activated