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Refolding of synthetic monobodies The polypeptides were dissolved in solubilization buffer (6 M guanidine-HCl, 500 mM arginine-HCl, 20 mM HEPES, pH 8.5, 150 mM NaCl, 0.5 mM TCEP-HCl) at a concentration of 0.5 mg/mL and transferred into a Slide-A-Lyzer G2 dialysis cassette with a cut-off of 3.5 kDa (Thermo Scientific)

Pretreatment of cells with these compounds prior to stimulation prevented I a degradation

Mohammed Shammakh for their generous assistance with data collection

Finally, based on the noted beneficial effect, BPC 157 may balance collagen synthesis and affect hepatic fibrosis, as a model of the wound-healing response to chronic liver injury, which was modified (Wynn, 2008

& Parkening, T

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