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Constitutive expression of the H86A mutant was approximately two times as high as WT in the absence of extracellular cysteine and remained at this level even when medium cysteine levels were increased to 1 mM

H., Monian, P., Quadri, N., Ramasamy, R

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Acrolein has also been noted to covalently modify the lysine and histidine residues of Cu/Zn-SOD, resulting in loss of enzymatic activity

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Prepare all reagents, working standards and samples as directed in the previous sections