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The plates were incubated for 2 h at 37C and the absorbance at 450 nm wavelength, which was obtained as the optical density (OD) value, was measured with a Multiskan SkyHigh automatic microplate reader (Thermo Fisher Scientific, Inc.)

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doi: 10.1056/NEJMoa1109400 158 ObeidRAwwadHMRabagnyYGraeberSHerrmannWGeiselJ

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This observation aligns with the higher mitotic activity of the proximal region of the matrix, feeding the faster moving IRS layer with more cells as compared to the cortex, and this also aligns with the higher speed of the IRS layer cells relative to the cortex cells in the supra-bulbar region (Fig