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Slides were dried for at least 20 hours at 4C and fluorescent images were obtained with Slidebook 4.2 Digital Microscopy Software driving an Olympus 1X81 inverted confocal microscope with a 60X oil immersion lens, NA 1.4 and resolution 0.280 Dendritic spine density was measured on primary and secondary dendrites at a distance of at least 150 from the soma

These signals can shift gut movement, pain sensing, and barrier strength

Immunofluorescence and immunohistochemistry Testis tissues or TM3 cells were fixed and processed for immunofluorescent or immunohistochemical staining as described previously [35]

Nitric oxide plays a critical role in the recovery of Lewis rats from experimental autoimmune encephalomyelitis and the maintenance of resistance to reinduction

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