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The serum samples will be divided into microtubes and immediately frozen at a temperature of -80C

Abstract L-Carnitine is a bioactive compound derived from L-lysine and S -adenosyl-L-methionine, which is closely associated with the transport of long-chain fatty acids in the intermediary metabolism of eukaryotes and sought after in the pharmaceutical, food, and feed industries

In addition, acylcarnitine accelerates the degradation of the PD1 protein by promoting the acetylation of the E3 ubiquitin ligase FBXO38, revealing not only the mediating role of acylcarnitine in mitochondrial communication between platelets and B cells but also its epigenetic regulatory role in regulating mitochondrial function and PD1 degradation [43]

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This phenomenon can be reversed by l-Carnitine through attenuating the p47Phox level, which leads to the detection of a decreased cytosolic ROS level through the DCFDA reaction under a fluorescent microscope (Fig