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FIGURE 1 In the original analysis, classification appeared to be supported by functional data such as substrate selectivity and inhibition characteristics ( Discovery of Ketosteroid Isomerase In 1955 Talalay and Wang discovered a new enzymatic reaction involving ketosteroids, including 5-androsten-3,17-dione (5-AD) and 5-pregnen-3,20-dione (5-PD), and purified a highly active isomerase from Pseudomonas bacteria ( The enzyme, ketosteroid isomerase, obtained from several bacterial species has subsequently been extensively investigated by incisive structural and functional studies owing to its extraordinary catalytic efficiency (Figure 2)