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Direct injection of bone marrow derived stem cells into the frontal motor cortex (a brain region) of human ALS patients is generally safe and well tolerated (Martinez 2012)

This effect was particularly pronounced in MST6-I cells treated with secretome from TWAT(MST6-I) adipocytes [Supplementary Figure 2D]

The protein elution was concentrated with a 100-kDa cut-off concentrator (Millipore) and loaded onto a size-exclusion column (Superose 6 Increase 10/300 GL, Cytiva) equilibrated with buffer C containing 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM DTT, 0.06% digitonin (w/v)