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The following data were extracted from each article: Organism Origin of sample (including whether the study was in vivo or in vitro) Sample size Study design Resistance status of study organism (suspected resistance, confirmed resistance, confirmed reduced tolerance, susceptible) Mechanism of resistance (target-site/metabolic/efflux pump-related, gene location, corresponding mutation, or metabolite implicated in the resistance mechanism) Intensity of resistance The data were summarised in a table, and the methodology, setting, and results of each study were synthesised narratively

Kotz and N.L

Acid solution was aspirated and neutralized by washing with 0.1 M Borate buffer, pH 8.5 (Thermo Fisher Scientific) three times for 3 min each

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