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By enabling the optimization of treatment regimens, this model may serve as a valuable tool for improving drug safety and therapeutic outcomes

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Isolation of rat heart mitochondria Upon cervical dislocation of 912 weeks old male Wistar rats (Charles River, UK), heart mitochondria (RHM) were prepared by homogenization of their heart tissue in STEB buffer (250 mM sucrose, 5 mM Tris-HCl and 1 mM EGTA, pH 7.4) supplemented with 0.1% fatty acidfree BSA

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During this process, small atom clusters are released due to high voltage gaseous ions striking the target surface within an evacuated chamber