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Specifically, their findings demonstrate that, upon release from its inhibitor, Kelch-like ECH-associated protein 1 (Keap1), Nrf2 translocates to the nucleus, activating the expression of genes involved in antioxidant production and detoxification processes

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After observing leakiness of the GFP from the ER to the Golgi at the steady state, due to the presence of free biotin coming from DMEM/F-12 and B27-Supplement, we sequestered it by supplying 0.1 mg/ml of NeutrAvidin Protein (Thermo Scientific, #31000) to the WiBi media for 2-3 days before performing live imaging

And the increased IGF1 expression was observed to enhance phosphorylation of IGF1r

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