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Each lysate was supplemented with protease inhibitor cocktail (#P8340, Sigma-Aldrich), phosphatase inhibitor cocktail 2 (#P5726, Sigma) and phosphatase inhibitor cocktail 3 (#P0044, Sigma)

After washing, the slices were fixed with 4% PFA in PBS for 1 h at room temperature, and the fixation reaction was quenched with 100 mM NH 4 Cl in PBS for 30 min

We have all had a debilitating headache before where only a dark, quiet room and some rest offered any relief

However, the product itself, including its ingredients and capacity, remains exactly the same, so you can use it with confidence

*Store in a cool, dry place (protect from moisture)