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"Vitamin D is thought to be involved in the healthy production of neurotransmitters," Dr

This decline often leads to visible signs like wrinkles and physical signs like joint stiffness or slower recovery times after exercise

As an unsupervised statistical method, PCA can simplify the multivariate data into a few principal components, which can highlight intrinsic trends, distribution of clusters and the existence of outliers

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After treatment, cells were washed, tripsinized and resuspended in FACS buffer (PBS with 5% FBS and 1mM EDTA) and analyzed on an LSR Fortessa (BD Biosciences) with plate reader operated on BD FACSDiva software (v9.0)