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Figure 4 Table 2 Summary of studies investigating microbiota-targeted interventions in PCOS

Cells, particularly neurons, have developed sophisticated systems to regulate iron homeostasis through absorption, storage, and output, involving transferrin (TF)-transferrin receptor (TfR), ferritin (FT), and ferroportin (FPN) (Figure 1B

10.1007/s00441-016-2464-0 60 TaoY.AuperinA.SireC.MartinM.SaliouM

8.4 Recommended evidence-focused next steps for clinical development Below are pragmatic, evidence-aligned steps to advance OSCs from mechanistic promise toward human proof-of-concept, emphasizing verifiable endpoints and risk mitigation: Prioritize OSCs with measurable human PK/PD (e.g., sulforaphane) - Use SFN as a clinical test case to establish PD endpoints (Nrf2-target induction, cytokine panels) and to refine assay timing and sampling windows (0.58 h post-dose for plasma metabolites

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