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Evoking and enhancing ferroptosis of cancer stem cells by a liver-targeted and metal-organic framework-based drug delivery system inhibits the growth and lung metastasis of hepatocellular carcinoma

2.9.2 Animal models Female Balb/c mice (45 weeks) were purchased from Shanghai Laboratory Animal Center (SLAC, Shanghai, China) and bred in a sterilized, specific pathogen-free (SPF) Lab of Tongji University

The recombinant lentivirus was then used to transduce U87MG and U138MG cells, and selection was performed with incubation for 2 weeks with puromycin (1.5 g/ml), resulting in the stable-expressing luciferase glioma cell lines U87-Luc and U138-Luc

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For this type of staining paraffin-embedded sections were first de-parafinized at 60 C and then the sections were rehydrated by soaking them in solutions with decreasing concentrations of ethanol in water (100%, 95%, 70% etc.)

While the time frame of a single treatment varies from person to person, a session typically lasts 30 45 minutes