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Description

FSA is conducted for important metabolites such as ATP, NADPH, NO, succinate, and itaconate in normal (without turning off any pathways), glycolysis off and OXPHOS/TCA off condition

Purification of GST-tagged proteins For protein expression, 400 mL bacterial cultures (DH5 or BL21 Codon Plus RIPL) were transformed with the corresponding expression plasmids, grown in LB medium supplemented with appropriate antibiotics and induced with 0.1 mM IPTG at OD 600 = 0.81.0

Journal of Biomechanical Engineering 132(1): 011010

Chauhan D, Kumar R, Thakur N, Singh M, Kumar K

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