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The 1 ATPase mix (5 mM ATP, 3 U per ml of pyruvate kinase (Sigma), 3 U per ml of lactate dehydrogenase (Sigma), 1 mM NADH and 7.5 mM phosphonyl pyruvate (Sigma)) was incubated with the hs 26S proteasome (typically at 100 nM) in the presence or absence of FAT10 (10 M) and NUB1 (15 M) in reaction buffer

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et al., 1995