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( D ) Representative Western blot of intracellular and secreted type 2 signature markers following SLC7A11 chemical inhibition with erastin for 24 hours ( n = 34)

Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h

[PubMed 4722839] 99

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Research has established clear links between oxidative stress and various forms of hair loss, suggesting that glutathiones powerful antioxidant properties may help counteract these effects

Identification of GLS as a cuproptosis-related diagnosis gene in acute myocardial infarction [J]