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The next day, cells were washed with PBS and incubated with a secondary antibody (AlexaFluor 568 conjugated goat anti-rabbit IgG (ThermoFisher Scientific, 1:500) and Hoechst 33342 (ThermoFisher Scientific, 1:1000) in blocking buffer for 1 h at room temperature

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developed the asymmetric synthesis of R -ALA using an enantioselective aldol reaction as the key transformation