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Three-micrometer slices were subjected to hematoxylin and eosin (HE), immunofluorescence (IF), and immunohistochemical (IHC) staining

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Conversely, lifestyle modifications, including physical activity, Mediterranean or DASH (Dietary Approaches to Stop Hypertension) diets, smoking cessation, and stress management, have been shown to restore redox balance and reduce disease severity (Fogacci et al., 2019)

For explant assays, the tissue (SMG and PG) was incubated with 50200 nM CCh (200 nM CCh shown in results) for 4 h before being lysed for RNA

The multifaceted role of iron in renal health and disease

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