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Only those with intermediate affinity to self-peptide-MHC (pMHC) interactions are positively selected and further differentiate into single-positive (SP) CD4 + or CD8 + thymocytes ( Nfkbia -SR) or the constitutively active form of IKK ( Ikbkb -CA) ( Other studies that used transgenic mice expressing mutant IkB or a conditional deletion of IKK or NEMO/IKK have also showed that reduced NF-B signaling affects CD8 SP T cells beyond thymic selection ( NF-kB signaling intermediates upstream of the IKK complex are also important in thymic development