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These optimized conditions achieved a 33.77% (w/w) FBP extraction yield, and the purity of FBP was 90.82%

This difference arises in part from suppression of ionization by constituents present in the biofluid that is being analyzed and can lead to significant imprecision during quantitative analyses Citation[2]

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217 This effect is attributed to compensatory mTORC1 activity, characterized by increased expression of several upstream regulators of mTORC1 (e.g., IL-7R and JAK1) and activation of its downstream targets S6K1 and 4E-BP1