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Two modifications: R3 substitution (Glu3Arg3, IGFBP affinity reduced 100-1000x) and 13 aa N-terminal extension

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Here, we used the CAGF cloning method to find the GST fragments interacting with glutathione, which might be useful for the finding of GST inhibitors

Especially when using benzoyl peroxide, one should also avoid using other topical medicines which can interfere with this ingredients mode of action, within 1 hour before or after its application

The marginal cost difference between proper equipment and improvised alternatives is $8-15 per protocol