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Cell counting kit (CCK)-8 analysis KGN cells (2 103 cells/well, 100 L/well) were plated in 96-well plates and cultured overnight, followed by continued culture for 72 h for CCK-8 assay

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Takahara M, Takaki A, Hiraoka S, Adachi T, Shimomura Y, Matsushita H, Nguyen TTT, Koike K, Ikeda A, Takashima S, Yamasaki Y, Inokuchi T, Kinugasa H, Sugihara Y, Harada K, Eikawa S, Morita H, Udono H, Okada H (2019) Berberine improved experimental chronic colitis by regulating interferon-gamma- and IL-17A-producing lamina propria CD4(+) T cells through AMPK activation