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To assess whether macrocycles and hHGF interacted with human, mouse and canine Met, each biotinylated macrocycle or hHGF was immobilized to a surface density of 200500 RUs (for biotinylated macrocycles) or ~2,200 (for biotinylated hHGF) RUs on a CAP sensor chip using a Biotin CAPture Kit (GE Healthcare), following the standard protocol