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PLoS ONE 15:e0231173

Disclosures: David Lee: Nothing to Disclose, Mohammed Shaik: Nothing to Disclose, Sharmitha Yerneni: Nothing to Disclose, Ki Jung Lee: Nothing to Disclose, Andres Chavez-Barragan: Nothing to Disclose, Andrew Yi: Nothing to Disclose, Gregory Fan: Nothing to Disclose, Nathalie Urrunaga: Nothing to Disclose 1584 IDENTIFYING RISK FACTORS FOR RETURN TO ALCOHOL USE IN POST-LIVER TRANSPLANT: THE ROLE OF SOBRIETY DURATION AND AGE Natalia Rodriguez-Payan 1 Maria P Cote 1 , 1 Massachusetts General Hospital Background: While sobriety is most often the goal for alcohol consumption after LT, approximately 30-40% of LT recipients will return to alcohol use within the first 5 years post-LT, increasing the risk of recurrent alcohol associated liver disease, and death

Quantitative PCR was performed with the KAPA SYBR Fast qPCR kit (Kapa Biosystems, Wilmington, MA) on an Applied Biosystems StepOne Real-time PCR system (Foster City, CA)

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A study using IL-17A gene knockout and anti-Ly6G depletion of neutrophils confirmed that the IL-17Aneutrophil axis was the indirect cause of the enhanced intestinal inflammatory response driven by CS exposure (97), illustrating the crucial role of pulmonary immune homeostasis in the tolerance of the lungs to environmental stimuli in the gut