PMC 304885
This structural evidence establishes arr1 as an active regulatory protein rather than a passive scaffold and suggests a potentially general mechanism for arr-mediated signaling across diverse effectors
These are produced and maintained by goblet cells which renew the inner mucus layer approximately every hour (Johansson et al., 2011)
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Briefly, cells were grown in YPD until logarithmic phase, harvested by centrifugation at 1,000 g for 3 min at room temperature and resuspended in 200 l One-step-transformation buffer containing 40% polyethylene glycol 3,350 (Sigma Aldrich, St
Ponsero AJ, Igbaria A, Darch MA, Miled S et al (2017) Endoplasmic reticulum transport of glutathione by Sec61 Is regulated by Ero1 and Bip