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Reduction studies using standard laboratory reagents may be employed to evaluate structural stability and confirm the presence of the disulfide linkage

147 Additionally, in the middle cerebral artery occlusion (MCAO) stroke model of mice, Li + can significantly attenuate GSDMD-mediated glial pyroptosis by regulating the AKT/GSK3/TCF4/-catenin signaling pathway, in which, the activation of AKT induced by Li + can also increase the phosphorylation of FoxO3a and promote the transportation of FoxO3a from nucleus into cytoplasm, the reduced FoxO3a in nucleus dissolves its competition with TCF4 in order to confirm more -catenin/TCF4 complex

doi: 10.1016/j.nmd.2021.06.009