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10.1016/j.bpj.2009.08.003 29

Protein Purification by HisTrip Column and 6XHis Tag TEV Cleavage Frozen bacterial pellets were resuspended in the lysis buffer (50 mM Tris-HCl, 150 mM NaCl, pH 8.0), and the cells were lysed using an ultrasonic processor

Despite the wealth of information about the biological activities of GSH and GSHee, the possibility that GSHee has the ability to modulate any of the pharmacological effects of opioids has not been explored to date

We next compared whether ABHD11 inhibition or glutarate stress resulted in similar changes in lipoylation and glutarylation on ketoacid dehydrogenases